Regeneration
Stimulates deep tissue repair processes.
First booster system based on PDRN with proven effectiveness
TwAc
TwAc is a new generation of skin regeneration boosters, developed with the participation of international biologists and doctors from YUMA Medical LP.
Unique molecule Highest Concentration
The medium-molecular-weight of PDRN Most effective in regenerative processes. They actively stimulate the synthesis of collagen, elastin, and hyaluronic acid (HA), significantly improving skin turgor and the vascular network.
The most concentrated product on a market TwAc 3.0 60 mg PDRN and 60 mg HA, moreover due to hyaluronic acid the action of polynucleotides is significantly prolonged
The most concentrated product on a market TwAc 3.0 60 mg PDRN and 60 mg HA, moreover due to hyaluronic acid the action of polynucleotides is significantly prolonged
Production and purification Safety
Purification of PDRN is carried out in strict accordance with European standards at the HTL Biotechnology plant in France.
TwAc® is safe, hypoallergenic, fully biodegradable, and meets high international quality standards (ISO 13485)
TwAc® is safe, hypoallergenic, fully biodegradable, and meets high international quality standards (ISO 13485)
Preclinical and Clinical trials Regeneration
Pre-clinical trials of TwAc® have shown a significant increase growing vessels up to 173% just 21 days after the procedure, indicating a powerful angiogenic effect.
TwAc® In studies of collagen stimulation, the increasing of collagen fibers in different parts of the wound ranged from 14% to 26%.
TwAc® In studies of collagen stimulation, the increasing of collagen fibers in different parts of the wound ranged from 14% to 26%.
TwAc Product Line
The TwAc line includes three products — Eyes, 2.0, and 3.0 — all based on Polydeoxyribonucleotide (PDRN) and without lidocaine for maximum biocompatibility. They differ in concentration, viscosity, and indications to cover various zones and skin regeneration needs.
TwAc Eyes
TwAc 2.0
TwAc 3.0
TwAc Eyes (PDRN 10 mg/ml)
TwAc Eyes is a delicate booster created for the regeneration of the periorbital zone, improving the quality of the skin in the lower third of the face, and treating alopecia. The preparation is ideally suited for thin, sensitive skin where a mild effect without excessive volume is needed.
Indications:
Superficial wrinkles around the eyes, dark circles, puffiness, skin atony (loss of tone) in the lower third of the face, alopecia.
Injection depth:
Subdermal, 1–4 mm — directly under the wrinkle or into the area of puffiness, which reduces the risk of lumps forming and ensures a uniform effect.
Composition:
PDRN 10 mg/ml. The concentration is selected for superficial skin layers — it stimulates cellular regeneration while remaining light and malleable in the tissues.
Result:
Post-course, the eye area appears more elastic, brighter, and smoother, with reduced wrinkles and dark circles lasting up to 3 months.
Course:
3 procedures with an interval of 7 days.
Features:
Low-molecular solution with low viscosity, easily spreads in the superficial layers, does not create indurations (firming/nodules), and ensures comfort during administration.
Volume:
1 ml, needle 34G.
Delicacy and Periorbital Zone Restoration
TwAc Eyes is a delicate product created for improving the quality of the skin in periorbital zone, remove «dark circle» under eyes and eliminate puffiness.
TwAc 2.0 (PDRN 7.5 mg/ml + HA 12 mg/ml)
TwAc 2.0 is a universal medium-intensity booster designed to hydrate, brighten the skin, and prevent aging. The product is ideal for patients with combined aging zones where a moderate effect is needed without excessive volume.
Indications:
Skin dryness, fine wrinkles, uneven tone, skin atony (loss of tone) of the face, neck, décolleté, and hands.
Injection depth:
Subdermal 2-3 mm — using the papule or linear-retrograde technique into the dermis, which ensures uniform moisturizing and stimulation of regeneration.
Composition:
PDRN 7.5 mg/ml + HA 12 mg/ml. The concentration is selected for the middle layers of the skin — it maintains turgor and radiance, while remaining plastic in the tissues.
Result:
Post-treatment, skin appears elastic, smooth, and radiant, with fine wrinkles diminished and a natural, rested look lasting up to 3 months.
Course:
2 procedures with an interval of 14 days.
Features:
The medium viscosity of the solution allows the preparation to be uniformly distributed in the tissues, preventing indurations (nodules) and ensuring comfort during administration.
Volume:
3 ml, needle 30G.
Delicacy and Periorbital Zone Restoration
TwAc Eyes is applied to the periorbital zone, the lower third of the face, and the scalp. Its especially delicate formula with a low Polydeoxyribonucleotide (PDRN) concentration is designed for superficial injection into thin, sensitive skin. It provides pronounced hydration, reduces fine wrinkles (crow's feet), brightens dark circles, and stimulates local angiogenesis, yielding the best results when introduced superficially while minimizing the risk of swelling.
TwAc 3.0 (PDRN 20 mg/ml + HA 20 mg/ml)
TwAc 3.0 is a high-intensity booster designed for deep regeneration and tissue restoration. The preparation is ideally suited for areas requiring pronounced lifting and the formation of a skin framework without excessive volume.
Indications:
Deep wrinkles, atony, decreased turgor of the face, neck, décolleté, hands, and body.
Injection depth:
Subdermal 3-5 mm — at the border of the dermis and subcutaneous fat, which provides pronounced lifting and long-lasting regeneration.
Composition:
PDRN 20 mg/ml + HA 20 mg/ml. The highest concentration among the three products provides a shape-retaining effect, remaining homogeneous and not creating nodules upon injection.
Result:
After the course, the skin becomes denser, firmer, and smoother. Deep wrinkles are smoothed, tone is evened out, pigmentation is lightened, and the face looks rested and natural. The effect lasts up to 3 months.
Course:
2 procedures with an interval of 21 days.
Features:
The highly concentrated solution forms an internal framework, stimulates neocollagenesis, and supports tissues, preventing the formation of indurations and ensuring comfort during administration.
Volume:
3 ml needle 27G.
Deep Regeneration and Lifting
TwAc 3.0 is used for the face, neck, décolleté, hands, and body. This is the most potent booster in the line, intended for deep regeneration, intensive lifting, and the restoration of turgor in cases of significant loss of elasticity. Its high PDRN concentration ensures prolonged stimulation of collagenogenesis and the restoration of the dermal structural components. It is used for the correction of scars, stretch marks (striae), and skin recovery after aggressive procedures.
Proven efficacy supported by research
TwAc has a strong evidence base: histological studies assessing CD34-positive vessels, along with clinical observations and VAS questionnaires, confirm tissue regeneration, improved skin quality, and high patient satisfaction.
PDRN with ultra-high molecular weight ≥1,480 kDa
The Polydeoxyribonucleotide (PDRN) in the TwAc formulation has an ultra-high molecular weight of at least 1,480 kDa, placing it among the highest-molecular-weight PDRN products currently available on the market. This elevated molecular weight is considered particularly effective for regenerative processes, ensures pronounced biological activity, and supports intensive tissue repair and skin regeneration.
Prolonged effect thanks to hyaluronic acid and PDRN
In TwAc 2.0 and TwAc 3.0, hyaluronic acid forms a reservoir-like matrix that enables gradual Polydeoxyribonucleotide (PDRN) release into tissues, providing a prolonged effect with lasting hydration, turgor, radiance, and visible results even after fewer treatment sessions.
High safety and quality profile
Salmon-derived Polydeoxyribonucleotide (PDRN) in TwAc undergoes multistep purification, has low endotoxin levels, and is fully biodegradable. ISO 13485–certified manufacturing and full biocompatibility testing minimise the risk of inflammation, allergic reactions, and long-term complications.
TwAc product line: a differentiated approach to regenerative therapy
The TwAc range (TWAc Eyes, TWAc 2.0 and TwAc 3.0) has been developed as an integrated system of regenerative boosters that covers the full spectrum of clinical indications in aesthetic dermatology and regenerative medicine.
Comprehensive portfolio for different clinical needs
TwAc Eyes, TwAc 2.0 and TwAc 3.0 cover a full range of clinical indications—from periorbital care and preventive revitalisation to deep regeneration and lifting—enabling personalised, coordinated treatment protocols.
Mechanism of action of TwAc
Study of Skin Regeneration: Hematoxylin-Eosin Staining. Scalped Rat Skin Wound
TwAc has an ordered, dense Polydeoxyribonucleotide (PDRN) structure that distinguishes it from conventional revitalizing agents.
The illustration shows that in older boosters, the molecules are arranged chaotically, while in TwAc, they are uniformly and structured, forming a stable network. This homogeneous matrix ensures smooth injection without needle blockage and even distribution within the tissues. As a result, TwAc maintains activity longer, is less susceptible to enzymatic breakdown, and demonstrates a more stable clinical outcome.
7 Days
NaCl 0.9%
Control Group
In the TwAc group, the total number of newly formed CD 34-positive vessels is greater.
14 Days
NaCl 0.9%
Control Group
Polynucleotides
In TwAc animals, the vessels are more numerous and dilated.
21 Days
NaCl 0.9%
Control Group
Polynucleotides
Vessel density in the TwAc group is higher compared to the reference group.
Vessel Growth: Comparison with Competitors
Vessel Growth Dynamics
The graph shows the dynamics of CD34-positive vessel growth for the TwAc® and control groups. The vertical axis represents the number of vessels per $1\text{ mm}^2$, and the horizontal axis represents the observation days (7, 14, 21).
Key TwAc Advantages
The TwAc curve is consistently higher, indicating faster and more intensive recovery. The growth is uniform, without drops, whereas a slowdown is observed in the control groups.
Stable and Predictable Outcome
The beginning of the TwAc curve is steep, pointing to the rapid activation of regeneration processes. This confirms the high effectiveness of the preparation: recovery is more precise, healing is faster, and the result is more stable and predictable.
Biological Action of TwAc
Innovative Impact of TwAcon the Skin
Healing
Accelerates re-epithelialization (renewal of the skin’s outer layer).
Energy
Enhances the metabolic activity of cells.
Microcirculation
Stimulates angiogenesis (formation of new microvessels).
Hydration
Enhances the moisturizing effect of hyaluronic acid in cells.
Inflammation Control
Reduces levels of pro-inflammatory cytokines (TNF, IL-12).
Antioxidant Protection
Acts as a highly effective "trap" for free radicals.
Even Tone
Has anti-melanogenic properties (prevents pigmentation).
Safety and Purity
Innovative effect of TwAc on the skin
Hypoallergenic
Genetic Safety
0% Toxicity
Apyrogenic
